Clone Development
We can provide services from gene synthesis to characterized cell banks. We undertake a milestone based approach with definite deliverables from gene cloning to selection of final clonal cell line.
Clone development services for microbial and mammalian cells include:
Microbial
- Gene synthesis, codon optimization and cloning into suitable vectors
- Multiple hosts screening for identification of suitable host strain
- Expression & productivity analysis
- Stability analysis of selected clones
- Cell banking and characterization
Mammalian
- Synthesis of codon optimized genes.
- Cloning in suitable expression vectors
- Confirmation of protein expression by initial transient transfection trials
- Transfection and stable pool generation using suitable selection and amplification
- Clonal selection by limiting dilution and screening of clones
- Productivity and stability analysis of selected clones
