Cell Line Development & Analysis

Syngene can generate homogenous stable clones in different cellular backgrounds including very hard-to-transfect cell lines and primary cells, for expressing genes of interest at high levels for reagent generation, assay development and biotherapeutic development.

We specialize in the over-expression of tagged and untagged proteins, G-protein coupled receptors receptor/ ligand, ion channels, micro RNA target genes, transcription factors and reporter genes. The activities include cell transfections for generation of recombinant cell lines, optimization of transfection, serum free adaptation, media optimization, cell line characterization, monoclonal antibody production and research cell banking.

Our cell engineering capabilities extend across knock-out (CRISPR-Cas9 & TALEN), knock-down (siRNA, shRNA) and knock-in (CRISPR-Cas9) in cell lines and primary cells. We also have expertise in genome-wide screening for target identification.


Cell culture capacity

  • Adherent: 96 well plate to Cell stacks
  • Suspension: 30ml to 3 L Spinners
  • Scale of expression: 2 – 10 L lab scale, 1-25 L Wave bioreactor and 50 – 300 L pilot scale

Cell line characterization

  • Real time PCR (96 and 384 well format)
  • Western blot
  • Flow cytometry (FACS)
  • Functional cell-based assays in 384/96 formats
  • Immunoassays
  • Immunofluorescence
  • Immunocytochemistry

Cell banking

Cell banking plays an important role in establishing cell stocks. In case of microbial contamination of routinely used cells, cryopreserved cells assure a back-up as well as continuous supply. Our services include expansion of cells for cryopreservation, viability testing, preparation of Research Cell Bank (RCB), and sterility and mycoplasma testing.


Infrastructure

Syngene has a sophisticated biology facility with high-end instrumentation. We have an experienced team with proven track record of supporting discovery and development programs under biology capabilities.

Some of the key instruments are listed below:

Cell Biology

  • FACS Calibur
  • FACS Accuri
  • FACS JAZZ
  • Clone Pix
  • Gene Pulser Xcell
  • Amaxa 4D Nucleofector
  • Biosafety Cabinets- BSL II
  • CO2 Incubators & Shakers
  • Phase Contrast & Fluorescence Microscopes (Apotome)
  • Spinner Flasks
  • Wave Bioreactor
  • Lab Scale Bioreactors
  • Automated Cell Counter
  • -156°C cryo freezer
  • Flow Cytometer (BD)